The Research

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The Research

Research and Publications

Pruvin’s efficacy has been demonstrated through decades of research conducted at Baylor College of Medicine in Houston, Texas. These clinical trials and animal studies have been primarily funded through highly competitive federal medical research grants. The results of this award-winning research have been widely presented within the medical community, including at the 2011 Annual Meeting of the American Diabetes Association. The conclusions drawn in these studies have passed the independent review panels of four peer-reviewed medical journal publication boards, and the scrutiny of experts at the United States Patent Office.

Publication Summaries

Impaired mitochondrial fatty acid oxidation and insulin resistance in aging: novel protective role of glutathione.

Nguyen D, et al. Aging Cell 2013;12:415-425. doi: 10.1111/acel.12073.
first-chartCompared with young humans, GSH-deficient elderly humans had 92% higher plasma fatty acid concentrations and 160% higher insulin resistance as measured by HOMA-IR. GSH restoration with the active ingredients in Pruvin™ completely restored fatty acid oxidation to values seen in younger controls. This was associated with 32% lower plasma insulin concentrations, 36% lower fatty acid concentrations and 40% lower insulin resistance. This “…holds the promise of a novel, practical, and innovative nutritional strategy to combat insulin resistance, obesity, and hepatic fat accumulation in elderly humans…” and therefore reduce risk factors for heart disease and diabetes.

Effect of increasing glutathione with cysteine and glycine supplementation on mitochondrial fuel oxidation, insulin sensitivity and body composition in older HIV-infected patients.

Nguyen D, et al. Journal of Clinical Endocrinology and Metabolism 2013;99:169-77. doi: 10.1210/jc.2013-2376.
second-chartThis study shows that HIV-infected patients >50 years old (as defined by the CDC) are unable to make enough GSH. Supplementation with cysteine and glycine (Pruvin) resulted in a 53% higher intracellular concentration of GSH along with improvements in fat oxidation, insulin sensitivity, body composition, and increased muscle strength. Plasma LDL-cholesterol and fatty acids (NEFA) decreased significantly. Insulin resistance improved as patients had a 16% higher glucose disposal rate and 31% increase in the insulin-sensitivity index. In the fasted state, they had a 46% increase in NEFA oxidation and a 49% decrease in carbohydrate oxidation. With a rise in GSH, plasma concentrations of toxic reactive oxygen species (ROS) and F2-isoprostanes (markers of tissue damage from ROS) also decreased significantly. Patients had a striking decrease in BMI, waist circumference (1 cm), fat mass (3.5 lbs. loss), and higher fat-free mass (1.98 lbs.). Forearm muscle strength gains suggest an improvement toward their chronological age by 10 years within a 2-week timeframe. Correction of GSH deficiency by dietary supplementation of these two amino acids improves the ability of the mitochondria to burn fat and carbohydrate more efficiently. This is also associated with improvements in insulin sensitivity, body composition (decreased fat and increased muscle) along with increased muscle strength.

Deficient synthesis of glutathione underlies oxidative stress in aging and can be corrected by dietary cysteine and glycine supplementation.

Sekhar RV, et al. American Journal of Clinical Nutrition 2011;94:847-53. doi: 10.3945/ajcn.110.003483.
Compared with younger control subjects, elderly subjects had markedly lower intracellular glutathione (GSH). Supplementation with cysteine and glycine (Pruvin) in elderly subjects for 14 days stimulated GSH synthesis (231% higher) to rates comparable with those of younger subjects and restored intracellular concentrations leading to significantly lower plasma oxidative stress (circulating ROS) and F2-isoprostanes. This approach in the elderly fully restores GSH synthesis and concentrations and lowers levels of oxidative stress and tissue damage to those of younger controls. It suggests a practical and effective approach to decreasing oxidative stress in aging with promise for the treatment of chronic conditions and diseases in elderly humans that are linked to increased oxidative stress.

Glutathione synthesis is diminished in patients with uncontrolled diabetes and restored by dietary supplementation with cysteine and glycine.

Sekhar RV, et al. Diabetes Care 2010;34:162-7. doi: 10.2337/dc10-1006.
Compared to controls, diabetic subjects had higher fasting glucose, lower glutathione (GSH) synthesis rates and lower intracellular GSH concentrations. Following dietary supplementation with GSH precursor amino acids (Pruvin) in diabetic subjects, GSH synthesis and concentrations increased significantly and plasma oxidative stress and lipid peroxides (markers of oxidative tissue damage) decreased significantly.

The results of this study are significant for several reasons. First, the study identifies deficient synthesis as a key reason for GSH deficiency in diabetes predisposing to elevated oxidative stress and oxidative damage. Second, the study identifies a simple, effective and safe approach, Pruvin, to reverse GSH deficiency, oxidative stress, and oxidative damage. Third, in the field of diabetes, the success of almost every intervention depends on glycemic control. Glucose per se is not toxic, but promotes tissue damage by increasing production of toxic free radicals (oxidative stress). Pruvin directly targets oxidative stress, corrects it, and limits further tissue damage. Pruvin would work perfectly with the current approaches and therapies.